Reveal three cryptic speciesin Allgemeine Publikationen 03.01.2016 18:21
von Jean Haxaire
Two species complexes within the genus Xylophanes are addressed using a combination of morphological study and analysis of DNA barcode sequences. The existence of two and three cryptic species respectively within the X. loelia and X. neoptolemus complexes is revealed following consideration of both adult habitus and genital morphology, and the results of a phylogenetic analysis of partial COI sequences —DNA barcodes— for 38 specimens. The taxonomic status of the available names is discussed and to clarify and stabilize the confused nomenclature of this group, a neotype for Sphinx neoptolemus Cramer, 1780, and lectotypes for Choerocampa loelia Druce, 1878 and Chaerocampa trilineata Walker, , are designated. We describe three new species: X. lolita n. sp. Vaglia and Haxaire; X. balcazari n. sp. Haxaire and Vaglia; and X. cthulhu n. sp. Haxaire and Vaglia. The first is endemic to southeastern Brazil and closely allied to X. loelia; the second two are relatives of X. neoptolemus, of which the first is known only from Guerrero and Michoacán states in Mexico while the second is widely distributed in lowland forests of Central America.
Xylophanes Hübner,  is the most speciose genus of the family Sphingidae (hawkmoths or sphinx moths), with 96 valid species and subspecies names listed in the most recent checklist (Kitching & Cadiou 2000). The present study focuses on a species complex that comprises taxa generally treated as X. neoptolemus (Cramer, 1780) and X. loelia (Druce, 1878) and represents a first step toward the elucidation of a larger complex that also includes X. libya (Druce) and X. pearsoni Soares & Motta. In their current sense, both X. neoptolemus and X. loelia are relatively common species widely distributed across Central and South America. However, it has become apparent, from variation in wingspan and subtle differences in wing pattern an genital structures that these names refer to a complex comprising more than two species. These initial observations are further examined and presented in this paper, and used in association with genetic data to distinguish and describe three new species within this complex. The DNA sequences used are part of a larger project that is assembling DNA barcodes—a part of the COI mitochondrial gene—for all species of sphingids (see http://www.lepbarcoding.org). One of the key features of this global campaign is the strong involvement of expert taxonomists and the integration of genetic data within a traditional taxonomic approach. The association of taxonomic expertise with DNA barcoding results in an efficient and reliable identification tool applicable to any life stage. Simultaneously, the taxonomists benefit from an additional dataset by which to explore and describe diversity. This latter point is of special relevance to the discovery and characterization of cryptic species, whose general importance in biodiversity studies has recently received renewed emphasis (Bickford et al. 2007; Pfenninger & Schwenk 2007). DNA barcoding has already been used to uncover cryptic diversity in sphingid moths (Hajibabaei et al. 2006), but here the authors did not propose the formal description of any new species, reserving the task to expert taxonomists who would combine a traditional taxonomic approach with genetic results.
Material and methods
A total of 138 specimens in the collections of TV and JH formed the basis of this study, including 64 specimens of X. loelia from across the distribution range of this species. Of these 64, two individuals from Brazil (Pote, Minas Gerais), though closely resembling X. loelia in habitus, displayed an atypical wingspan and aslightly different wing shape. Seventy-four specimens of X. neoptolemus were also examined, including specimens that had been separated into three preliminary groups based on subtle differences in wing pattern and shape. For both species, an extensive survey of the variation in male genitalia was undertaken, with 63 specimens being dissected (28 X. loelia, 35 X. neoptolemus). We examined the type specimens of Choerocampa loelia Druce, 1878, and Chaerocampa trilineata Walker, , preserved in the BMNH, and the type of Xylophanes heinrichi Closs, 1917, preserved in the ZSM. C. trilineata and X. heinrichi were listed by Kitching and Cadiou (2000) as junior subjective synonyms of X. neoptolemus and X. loelia respectively, and we here confirm those synonymies. We failed to locate the type material of Sphinx neoptolemus in any of those major institutions known potentially to have types of species described by Cramer in their collections (MNHN, BMNH, RMCA, ZMA, NNMN). Thus, the only evidence available relating to the type(s) of X. neoptolemus is the short original description and the accompanying painting (Cramer, 1780: plate 301, fig. F). The plates in the various published copies of Cramer (1780) were copied by various artists from original paintings (sometimes referred to as the “pattern plates”,preserved in the Entomology Library of the BMNH) and although they are often somewhat stylized, the species represented are often clearly identifiable (Vane-Wright 1975; Chainey 2005). Nevertheless, the original paintings should always be consulted when making particularly critical determinations, as it is expected that these would generally be better matches for the type specimens than any of the published versions (Chainey 2005). We reproduce here in Fig. 1b the original painting of X. neoptolemus. Unfortunately, it is too imprecise to assess the subtle interspecific differences in this species complex. The falcate apices of the wings are striking at first sight, and it is a useful character in this group; however, as most of the illustrations by Cramer (1780) show such exaggerated acute apices, it is reasonable to question the accuracy of the representation of this character, which is more likely to have been due to the artistic considerations of that time than an accurate illustration of a specific morphological feature. Moreover, the abdomen lacks the typical longitudinal dorsal bands found in the neoptolemus complex, and as such the painting is more reminiscent of the pattern seen in X. loelia. Finally, the type locality, “Suriname”, is insufficiently precise to assist in determining the identity of the illustrated specimen. Therefore, we judge the illustration (Fig. 1b) to be an inadequate representation of a reliable type specimen of X. neoptolemus, and we therefore consider it necessary to designate a neotype to clarify and stabilize the nomenclature of this group (see below).
We have studied the holotype of Chaerocampa [sic] brasiliensis Schaufuss, 1870, another junior synonym of X. neoptolemus. (Note that Chaerocampa is an incorrect subsequent spelling of Choerocampa Duponchel that was widely used by many nineteenth century entomologists.) It is most likely to be in the ZIRAS, the only institution reported to date as having a type of a sphingid species described by Schaufuss from the Kaden collection (Cadiou 1995), but preliminary searches have so far failed to discover it. The description of Schaufuss (1870) is not detailed enough to allow the safe assignment of that name to any of the taxa treated herein. Moreover, we consider the stated type locality of C. brasiliensis, “Br.” (for “Brasilia”; that is, Brazil the country rather than Brasilia the city), to be meaningless because several other species referred by Schaufuss (1870) as coming from “Brasilia” have geographical ranges that do not intersect with even the broadest possible interpretation of this locality. For example, Schaufuss reported ‘Brasilia’ as a locality for Sphinx oestri (i.e. Manduca sexta caestri (Blanchard), endemic to Chile) and S. celeus (i.e., M. quinquemaculatus (Haworth), a species distributed from Canada to Guatemala). Chaerocampa brasiliensis must therefore be considered a species inquirenda (a species of doubtful identity requiring further study). We choose not to designate a neotype of C. brasiliensis because intensive searching of the ZIRAS collections may yet uncover the holotype and we do not want to restrict future options. So as not to clutter the taxonomy and nomenclature with dubious
species, we elect for now to follow Kitching and Cadiou (2000) and maintain C. brasiliensis as a junior synonym of Xylophanes neoptolemus.
The holotype of Xylophanes trinitatis Closs, 1917 was studied by Kitching and Cadiou (2000), who concluded that it was also a junior synonym of X. neoptolemus; the findings presented herein, as well as the type locality of X. trinitatis (Trinidad), strongly support this decision, and re-examination of this type beyond examination of a color photograph was deemed unnecessary for the present work. The relatively confused nomenclatural situation in this group, together with the need to fix the identities of some of the available names, requires that, in addition to a neotype for Sphinx neoptolemus, lectotypes for Chaerocampa loelia and C. trilineata be designated (see below). These, together with the holotype of X. heinrichi, are illustrated in the present paper.
Tissue samples (dry legs) were collected from 37 specimens (dry mounted moths) held in the collections of JH and TV, comprising eight specimens of Xylophanes loelia and 29 of X. neoptolemus, and including the morphological variants expected to represent new species. Among the data gathered in the context of the global DNA barcoding campaign for sphingids, we selected and used the sequences of three closely related taxa as outgroups: X. libya, X. aglaor (Boisduval) and X. cyrene (Druce). Details of each specimen are given in Table 1, and are also available within the projects ‘Sphingidae - Type specimens’ (code SPTYP), ‘Sphingidae - Haxaire collection PUBLISHED records’ (code JHPUB) and ‘Sphingidae - Vaglia collection PUBLISHED records’ (code TVPUB) in the Published Projects section of the Barcode of Life Data systems (BOLD; Ratnasingham & Hebert 2007; www.barcodinglife.org). Information on specimen vouchers (images, field data, GPS coordinates) and sequences (nucleotide composition, trace files) are found in these projects by following the ‘view all records’ link and clicking on the ‘specimen page’ or ‘sequence page’ links for each individual
Tissue samples were processed at the Canadian Centre for DNA Barcoding (CCDB). DNA was extracted from dry legs using a routine silica-based 96-well extraction automation protocol (Ivanova et al. 2006). The 658bp region of COI proposed for use as a ‘DNA barcode’ (Hebert et al. 2003) was amplified with the primer set LepF1/LepR1 (Hebert et al. 2004). The DNA extracts that did not amplify for the full-length DNA barcode were individually selected, reprocessed and re-amplified with the LepF1/EnhLepR1 (Hajibabaei et al. 2006) primer pair, targeting a 612 bp fragment of COI. All PCR amplifications were performed according to the standard PCR reaction protocol used in CCDB (Hajibabaei et al. 2005). PCR products were checked on a 2% E-gel ® 96 Agarose (Invitrogen). Unpurified PCR fragments were sequenced in both directions using LepF1, LepR1 or EnhLepR1 primers depending upon the one used in the PCR reaction. The sequencing reactions followed CCDB protocols (Hajibabaei et al. 2005), with products subsequently purified using Agencourt® CleanSEQ protocol (Agencourt, Beverly, MA, USA). The sequences were managed in SeqScape version 2.1.1 (Applied Biosystems, Foster City, CA, USA) and Sequencher 4.5 (Gene Code Corporation, Ann Arbor, MI, USA) and aligned using Bioedit version 22.214.171.124 (Hall 1999) and MEGA4 (Tamura et al. 2007). For specimens that did not amplify after this procedure, DNA extraction was re-done in isolated tubes using a commercial extraction kit (NucleoSpin® tissue kit, Macherey-Nagel, Düren, Germany) and following the kit protocol. The same sets of primers were used as well as the additional primer pairs LepF1/MLepR1 and MLepF1/LepR1 targeting shorter DNA fragments and usually successfully amplifying specimens whose DNA was degraded with a high success rate (Hajibabaei et al. 2006). Regularly updated protocols used at the CCDB can be found at: http://www.dnabarcoding.ca/pa/ge/research/protocols.
TABLE 1. Details of the 41 specimens used in the genetic analysis. L = sequence length; Dep. = depository collection (DH&WH = collection of Dan Janzen and Winnie Hallwachs, University of Pennsylvania); F = female; M = male. Alt. altitude in meters. SampleID and ProcessID are unique identifiers referring respectively to the voucher specimen and sequence information on BOLD. Holotypes of the species described in the present paper and the designated neotype of Xylophanes neoptolemus are in bold.
In addition, we included in our analyses a sequence of Xylophanes loelia from the study of Hajibabaei et al. (2006) in the context of the biodiversity inventory of Lepidoptera in the Area de Conservation Guanacaste, Costa Rica (http://janzen.sas.upenn.edu). This sequence and specimen data are accessible on BOLD in the published project ‘Sphingidae of ACG1’ (code MHASA); it was chosen among the 34 identical barcodes already available for this species from ACG. It should be noted that only a single specimen of X. neoptolemus from Costa Rica was included in the present work as no significant genetic variation has been observed within the 28 full length barcodes also available from the ACG survey (these sequences exhibit a maximum K2P distance of 0.6 %, as implemented in BOLD). References to DNA barcode records in BOLD are given throughout the text in the following format: SampleID/ProcessID (e.g. VAG-###/SPTVA###), where SampleID and ProcessID are unique identifiers linked to the voucher specimen and to DNA data respectively.
Phylogenetic analysis of DNA sequences and calculation of genetic distances Barcode sequences were first explored using the Neighbor-Joining reconstruction method implemented in BOLD, and further analyzed in a phylogenetic context using NONA 2.0 (Goloboff 1999) run from within WinClada 1.00.08 (Nixon 2002). A heuristic search using a branch swapping algorithm was performed using the following parameters: hold 100000, hold/100, mult*500 followed by max*. Branch support was estimated using the bootstrap, Bremer support (Bremer 1994) and rescaled Bremer support (RBS; Goloboff & Farris, 2001). Bootstrap values were calculated with 1000 pseudoreplicates analyzed using the same set of heuristic parameters given above. Bremer support and RBS were calculated using the commands ‘bSupport’ and ‘bSupport*’ in NONA following suboptimal tree searches. Among the selected outgroup taxa, Xylophanes cyrene is the most distantly related species and was chosen as the primary outgroup to root the trees. Distance calculations were performed using the Kimura 2-parameter (K2P) algorithm in MEGA4 (Kimura 1980; Tamura et al. 2007), including all sites with the pairwise deletion option, and assuming both a homogeneous pattern of divergence among lineages and a uniform rate of substitutions among sites.
Differential morphological patterns—taxonomic diagnosis
Both Xylophanes loelia and X. neoptolemus were found to encompass divergent lineages with consistent, though subtle, morphological differences in both the habitus and male genitalia. In particular, the two specimens of X. loelia from southeastern Brazil (Pote, Minas Gerais; Figs. 5a, 5b) differ from typical X. loelia (Figs. 6a, 6b) in wingspan and wing shape: they are significantly larger that all other specimens studied including X. neoptolemus) and have somewhat more elongated and less falcate wings. They are also unique in having a larger black discal spot on the forewing upperside and in the basal area of the hindwing upperside being black rather than the dark brown of X. loelia. Also, the pale median band of the hindwing upperside lacks the pinkish coloration typical of loelia. A closer examination of wing pattern details shows that the position of the oblique postmedian lines of the forewings is also different between these Brazilian specimens and other representatives of X. loelia: the first four lines are evenly spaced in the former, as they are in X. neoptolemus sensu lato, whereas the second and third are closer to each other than they are to the first and fourth respectively in X. loelia.
The X. neoptolemus complex was divided into three groups based on morphological studies. These groups are clearly separated geographically: the first (group 1) occupies lowland rain forest areas in South America (Figs. 3a, 3b) from northwestern Venezuela to French Guiana; the second (group 2) is restricted to the dry areas of Guerrero and Michoacán states in Mexico (Figs. 2a, 2b); and the third (group 3) is widely distributed in Central America (Figs. 4a, 4b) from Panama to southern Mexico. A number of differences were found among these three populations. In particular, specimens of group 3 can be immediately differentiated from the others by their large size, more brightly colored general appearance (especially the red median band of the hindwing upperside), the strongly falcate apex to the forewings and, on the underside, by a striking golden transverse band crossing the median area of the forewing. In contrast, specimens of group 2 have shorter and more rounded wings, as well as a different, paler coloration; the red median band of their hindwing upperside is significantly broader than in specimens from other geographical origins. All moths in group 1 are characterized by a very distinct dashed postmedian line on forewing and hindwing underside, highlighted by black vein dots.
The genital morphology of the species within the loelia/neoptolemus complex, and to some extent, within the larger complex including X. libya and X. pearsoni, is highly conservative and homogeneous. Consistent differences between species do exist, but they are very slight and subtle, requiring an attentive and meticulous comparative study based on a series of specimens to appreciate their value as species-specific differences rather than intraspecific interindividual variations. The male genitalia of the two specimens from southeastern Brazil (Figs. 9a–c) are very different in general appearance from those observed in other X. loelia specimens (Figs. 8a–c). Their general aspect is closer to that of X. neoptolemus sensu lato, but they are unique in the complex in having a much more massive harpe (Fig. 9b). The uncus (Fig. 9a) is thicker than that of typical X. loelia (Fig. 8a) but almost as straight as in that species.
Within the X. neoptolemus complex, most differences concern the shape of the uncus and the harpe. The setigerous lobes of the uncus are more developed in specimens from Central America (group 2 and 3, as defined above; Figs. 11a, 12a), though less markedly in specimens from group 2. The latter have a slightly spatulate stout uncus apex (Fig. 11a), whereas it is thinner and strongly spatulate in group 1 (Fig. 10a). Except for the slight difference in the development of the setigerous lobes, the shape of the uncus is very stable across Central America (groups 2 and 3), though it is somewhat stouter and more bent ventrally in group 3 (Fig. 12a). The harpe is bent in specimens of groups 1 and 2, whereas in specimens of group 3 it is regularly curved and narrowing gradually to taper to a delicate and slightly bent apex (Fig. 12b). Some minute diagnostic features were also observed on the apical processes of the aedeagus; the right lobe is wider in specimens from group 2 and 3 (Figs.11c, 12c), being especially stout in group 3. In this distinct population, the teeth of this lobe are long, evenly sized and mostly concentrated at the apex of the lobe (Fig. 12c). Specimens from group 2 show more unevenly distributed teeth (Fig. 11c).
Molecular divergence and species-level phylogeny
All of the 37 samples were eventually successfully sequenced using the different amplification procedures (see Material and Methods section), comprising 35 “full-length” barcodes (more than or very close to 600 bp) and two partial (307bp) sequences from the LepF1/MlepR1 primer pair (see Table 1). Phylogenetic analyses were computed for both a dataset reduced to full-length sequences and for the complete dataset including the two short sequences, with alignment gaps treated as missing data. We observed no deleterious effects of these short sequences on the final result. The dataset includes 64 parsimony informative characters (60 within the ingroup) and all observed substitutions are synonymous. The phylogenetic analysis resulted in nine most parsimonious trees, the strict consensus of which is shown in Fig. 13. Xylophanes loelia and X. neoptolemus are divided respectively in two and three strongly supported clades (bootstrap values ranging from 96 to 100%; Bremer support and RBS values ranging from 3 to 11 and 58 to 87 respectively). The genetic distances were calculated from the complete dataset; within and between clade distances are shown in Table 2. Intra-clade distances range from 0 to 0.2%, whereas inter-clade distances range from 2.6% (between the two loelia clades) to 6% (excluding outgroup taxa).
Discussion and species descriptions
The congruence in the discrimination of cryptic lineages by both morphological studies and phylogenetic analysis of the COI sequences is remarkable, and the perfect corroboration of the COI phylogeny by independent morphological features excludes any potential bias related to incomplete lineage sorting for this particular dataset. The Xylophanes loelia/neoptolemus complex thus appears to be a complex of five distinct species, which, though initially “cryptic”, are here clearly distinguished both morphologically and genetically. Taking the current nomenclatural and taxonomic status of the available names for this group into consideration, we describe below three new species and provide brief comments about the two previously valid names, loelia and neoptolemus, as well as their synonyms as listed by Kitching and Cadiou (2000).
FIGURES 1–4. 1a. Chaerocampa trilineata Walker, , Lectotype, m , Venezuela; 1b. Watercolour of the original type of Sphinx neoptolemus Cramer, 1780; 2a and 2b. Xylophanes balcazari n. sp., Holotype, m , Mexico, Guerrero; 3a and 3b. Sphinx neoptolemus, Neotype, m , Venezuela, Aragua; 4a and 4b. Xylophanes cthulhu n. sp., Holotype, m , Guatemala, Izabal. (2a, 3a, 4a: dorsal view; 2b, 3b, 4b: ventral view).
TABLE 2. Mean Kimura 2-parameter distances (%) calculated within (shaded cells) and between each clade/species resulting from the analysis of the complete DNA barcode dataset. Standard error estimates are shown within brackets and were obtained by a bootstrap procedure (500 replicates) as implemented in MEGA4.
Xylophanes loelia (Druce, 1878)
(Figs. 6a, 6b, 8a–c)
Xylophanes heinrichi (Closs, 1917)
Taxonomic Notes: Xylophanes heinrichi (Fig. 7a) was described by Closs (1917) from a single male collected by E. Christeller on 11.???.1912 (the month is unclear on the label) from “Amazonas”. This is an imprecise type locality, as it could refer to parts of Brazil, Venezuela, Colombia and even Peru. Of these, Brazil is perhaps somewhat more likely than the others but this is still not at all certain. However, regardless of where in “Amazonas” the moth was captured, the locality would be within the distribution of what we infer here to be X. loelia. The holotype, preserved in the ZSM, is illustrated in Fig. 7a. X. heinrichi is currently treated as a junior subjective synonym of Xylophanes loelia. Choerocampa loelia was described by Druce (1878) from an unstated number of specimens from Chiriquí, Panama, collected by Arcé. Only a single specimen with appropriate data and labels has been found in the BMNH and thus to stabilize the nomenclature, we hereby designate this specimen as the lectotype. The lectotype and its labels are illustrated in Figs 6a and 6b.
Distribution: This species is widely distributed in Central and South America. It is known to us from southern Brazil and Paraguay, north through Peru, Ecuador, French Guiana, Guyana, Venezuela, Trinidad and Colombia, and into Central America, through Costa Rica and Nicaragua, to Belize. Genetic variation: Intraspecific variation for the part of COI we sequenced is very low (Table 2; Fig. 13, clade #2).
Xylophanes lolita Vaglia & Haxaire n. sp.
(Figs. 5a, 5b, 9a–c)
Type material: Holotype (Figs. 5a, 5b; in coll. TV, to be deposited in the Insectarium of Montréal, genital prep. #271104a, VAG-375/SPTVA739-07): m , Brazil, Minas Gerais, Pote, 540 m., 30.xi.2004, leg. Roney Alves dos Santos. Paratype (BC-Hax0781/SOWA788-06): 1 m , same data as holotype but 16.xi.2004.
Description: This species is described on the basis of combined evidence derived from morphology and genetic data (Fig. 13, clade #1; Table 2). Overall, it is very similar to X. loelia but is immediately distinguishable by its larger size, wing shape, and constant genital differences.
FIGURES 5–7. 5a and 5b. Xylophanes lolita n. sp., Holotype, m , Brazil, Minas Gerais; 6a and 6b. Chaerocampa loelia Druce, 1878, Lectotype, m, Panama, Chiriquí; 7a. Xylophanes heinrichi Closs, 1917, Holotype, m “Amazonas”. (5a, 6a, 7a: dorsal view; 5b, 6b: ventral view).
Male (Figs. 5a, 5b). Head and body: Upperside of head, base of thorax and tegulae pale brown. Dorsal part of thorax and abdomen greyish-beige, with 11 longitudinal lines of unequal width; median thoracic line dark brown, thin, extending onto all abdominal segments, where it is bordered by a pair of thicker grey-brown lines, only slightly contrasting with the dorsal abdominal ground color. A pair of barely visible, black dots present immediately after these lines at the junction of each abdominal segment. Abdomen laterally with two pairs of alternately pale grey and brown lines; latero-ventrally orange-beige, with a pair of black dots on each segment; ventrally with five longitudinal lines, three cream coloured, interspaced with two pale brown lines. Thorax laterally orange-beige; ventrally pale brown, as are the legs and labial palpi. Forewing upperside: General coloration pale brown and beige. The wing is divided into four distinct areas. Basally pale brown, slightly greyish; costal margin, especially along the discal cell, deep brown. Median area pale beige, its distal part becoming brown when approaching the costa, though less contrasted than the latter. Discal spot round and black. Full complement of six oblique postmedian and two submarginal lines present. First postmedian line brown, arising above the inner margin, a few millimeters from the wing base, straight, clearly defined, curving slightly toward the apex, but not reaching it (unlike the next three). Second postmedian line more diffuse and less contrasted. Third postmedian line almost as dark as the first, whereas the fourth is identical to the second, though thinner. These four lines parallel and evenly spaced. Fifth postmedian line wider and darker than others, followed by a somewhat similar, though narrower, sixth line. Between the fifth and sixth postmedian lines, the wing background color is slightly orange, especially close to the inner margin of the wing. First submarginal line diffuse and poorly defined; second submarginal line evanescent, only clearly visible near torus. Fifth and sixth postmedian lines and both submarginal lines reach the apex. Hindwing upperside: Basal area dark brown, almost black. Median band wide, beige reaching the apex, with an irregular anterior margin and a smooth, clearly defined, posterior margin; Submarginal band dark brown (though less dark that the basal area) extending from tornus to apex. Forewing underside: Like the upperside, divided in four distinct areas: from base to median area, pale brown to grey-brown; median area orange brown; apical part beige; submarginal part of outer margin grey-brown. Postmedian and submarginal oblique lines, as described for the upperside, are apparent. First postmedian line dark brown, thick basally and narrowing toward costal margin, where it is reduced to a very thin trace. The next three postmedian lines grey-brown, contrasting little with the orange brown colour of this part of the wing; third postmedian line with a large black somewhat triangular spot between veins Rs 3 and Rs 4 ; fourth postmedian line with a small black vein dot on M 1 . Fifth postmedian line also grey-brown but wider that the preceding and bearing a row of distinct black vein dots on M 2 to CuA 2. Sixth postmedian line mostly visible between apex and vein M 3 ; shortly beyond this vein, it disappears into the orange-beige ground coloration of the wing. First submarginal line clearly visible, almost as contrasted as the first postmedian line; the second submarginal line reduced to a suffusion of brown scales. Hindwing underside: Basally grey-beige, progressively becoming pinkish-orange towards the postmedian area. Median area crossed by two longitudinal pale brown lines, distal of which is an interrupted line with black vein dots. Submarginal band, from tornus to apex, dark brown though paler on the veins and towards the apex.
Female and pre-imaginal stages. Unknown.
Male genitalia (Figs. 9a–c).
Overall, very similar to the other species of the complex. Uncus (Fig. 9a) relatively short, with well developed and setose lateral setigerous lobes; posterior third narrowed and downcurved, apex curved and spatulate. Harpe (Fig. 9b) short, basally very wide, bending and narrowing medially and becoming narrow and thinly upcurved apically; ventral part, especially basally, irregular and setose. Aedeagus (Fig. 9c), as in most Xylophanes species, with a transverse apical process bearing two distinct lobes; right lobe short, recurved, of even width only tapering at the apex, with about 20 tiny even teeth along its ventral and lateral margins; left lobe poorly developed, reduced to a plate following the left lateral margin of the aedeagus, thinly serrate with only few short scattered teeth from medially to the apex, where the serrations are slightly longer.
Distribution: This species is so far only known from Pote in Minas Gerais state, Brazil. It is likely to be restricted to this part of southeastern Brazil, which is known for its high level of endemism.
Genetic variation: The COI sequences for the two available specimens are identical.
Xylophanes neoptolemus (Cramer, 1780)
(Figs. 3a, 3b, 10a–c)
Chaerocampa trilineata (Walker, )
Chaerocampa brasiliensis Schaufuss, 1870
Xylophanes trinitatis Closs, 1917
FIGURES 8–12. Drawings of diagnostic details of the male genitalia of Xylophanes loelia (Figs. 8a–c), X. lolita n. sp. (Figs. 9a–c), X. neoptolemus (Figs. 10a–c), X. balcazari n. sp. (Figs. 11a–c), and X. cthulhu n. sp. (Figs. 12a–c); a. uncus in lateral view, b. harpe, c. posterior end of the aedeagus.
Taxonomic Notes: As noted above, we failed to locate any type material for Sphinx neoptolemus in any of the major European museums that might have been expected to house it. Furthermore, the quality of the original painting is insufficient to determine the species that Cramer had before him. Although there is no taxonomic problem per se regarding Sphinx neoptolemus itself in “Suriname” (even if this term is used in its broader Eighteenth Century interpretation, which could include the West Indies), it may not represent the taxon we treat here as Xylophanes neoptolemus but another species of Xylophanes altogether. Thus, we do not consider the painting to be useful as a representation of the type of Sphinx neoptolemus, although it is essentially consistent with our concept of this species. Therefore, with the express purpose of clarifying the taxonomic status of Sphinx neoptolemus and fixing the type locality, we hereby designate the following specimen as neotype (Figs. 3a, 3b; in coll. JH, to be deposited in the BMNH, BC-Hax4339/SOWE440-07): % , Venezuela, Aragua State, Maracay, station Rancho Grande, 30-31.viii.1983, leg. J. Haxaire & J.-Y. Rasplus. No specimens were available to us from Surinam and so we chose one from a locality that was as close as was practicable. Diagnostic features separating Xylophanes neoptolemus from its closest relatives, X. balcazari n. sp. and X. cthulhun. sp., are given below in the descriptions of those two species and in the identification key.
Chaerocampa trilineata (Fig. 1a) was described from two specimens from “Venezuela” from the Dyson collection. In the original description, Walker (: 30) indicated that he had only males. However, we have located both specimens in the BMNH and found that one of the syntypes is actually a female. To stabilize the nomenclature, we hereby designate the male specimen (BMNH(E)#274441) as the lectotype. The lectotype and its original labels are illustrated in Fig. 1a. The pale blue-edged syntype label will be replaced with a purple-edged lectotype label. In addition to a pale blue-edged syntype label (which will be replaced with a pale blue-edged paralectotype label), circular locality label and a printed specimen register number label, the paralectotype female (BMNH(E)#274442) has a hand-written label stating “trilineata Wlk.” and a label comprising the words “CHÆROCAMPA TRILINEATA.” cut from a copy of Walker’s catalogue.
As noted above, we have been unable both to locate the holotype of C. brasiliensis and to determine the identity of the taxon. Therefore, we maintain C. brasiliensis as a synonym of X. neoptolemus pending discovery of type material.
Xylophanes trinitatis was described from a holotype male from Trinidad, deposited in the ZSM. We have examined a color photograph of the holotype and this, together with the type locality, confirms its synonymy with X. neoptolemus.
Distribution: Following the neotype designation above, our specimens from Venezuela and French Guiana represent Xylophanes neoptolemus (Fig. 13, group 1). Furthermore, our biogeographic results support the previous treatments of X. trilineata and X. trinitatis as junior subjective synonyms of X. neoptolemus. X. neoptolemus occurs in the lowland tropical rain forest of the northern part of South America. We know it from western Venezuela to French Guiana. In the latter country, it is only frequent in the so-called ‘zone 1’ region of St-Laurent and St-Jean-du-Maroni (Haxaire 1987), near the Surinam border. This moth becomes far less abundant southward. The furthest south it has been recorded is from the Jari Celulose S.A. landholding (Hawes 2005), between the Jari and Paru rivers, northwest of Monte Dourado in Pará state, Brazil, but it may not cross the Amazon river as it is not mentioned by Moss (1920) in his work on the sphingids of Belém.
Genetic variation: There is some diversity in the barcode region (Fig. 13, group 1) but intraspecific divergence is low, with the five different haplotypes differing from each other by only a single base pair.
Xylophanes balcazari Haxaire & Vaglia n. sp.
(Figs. 2a, 2b, 11a–c)
Type material: Holotype (Figs. 2a, 2b; in coll. JH, to be deposited in CNIN; BC-Hax0759/SOWA766-06): m, Mexico, Guerrero, road from La Salitrera to Vallecitos de Zaragoza, km. 45, 500 m., 15.viii.1992, leg. D. Herbin & J. Haxaire. Paratypes (in coll. JH): 7 m (5 with DNA Barcodes–BC-Hax0763/SOWA770-06, BC-Hax0764/SOWA771-06, BC-Hax4325/SOWE426-07, BC-Hax4326/SOWE427-07, BC-Hax4327/SOWE428-07), same data as holotype (one male to be deposited in the BMNH); 1 m Mexico, Michoacán, road from Villa Victoria to Coalcoman, Los Laureles, 1538 m., 25.vi.2008, leg. J. Haxaire, O. Paquit & G. Nogueira; 4 m , Mexico, Michoacán, Coalcoman, Puerto La Zarzamora, Cerro El Laurel, 1635m., 26.vi.2008, leg. J. Haxaire, O. Paquit & G. Nogueira; 2 m , Mexico, Michoacán, Coalcoman, Puerto La Zarzamora, Cerro El Laurel, 1635 m., 27.vi.2008, leg. J. Haxaire, O. Paquit & G. Nogueira; 2 m , Mexico, Michoacán, Coalcoman, Puerto La Zarzamora, Cerro El Laurel, 1635 m., 8.vii.2008, leg. J. Haxaire, O. Paquit & G. Nogueira.
Description: Male (Figs. 2a, 2b). Forewing length: 32 mm. Forewing upperside: General background color olive-beige. Discal spot small and black. Full complement of six oblique postmedian and two submarginal lines present, more or less easily distinguishable depending on specimen condition. First and fifth post- median lines the most obvious, broader than the others. First postmedian line straight, stopping 2mm from costa, whereas the other lines all curve apically towards the wing apex. Area between first and fifth postmedian lines pale beige, crossed by the thin second, third and fourth postmedian lines; second postmedian line barely distinguishable except in its most apical part; third and fourth postmedian lines bend strongly beyond vein Rs 4 , becoming blurred toward the apex of the wing, although they do clearly reach it. The strongly marked fifth postmedian line is slightly sinuous, convex from inner margin to vein M 1 and then slightly concave from there to apex. Between this band and the next, the wing becomes reddish. Fifth and sixth postmedian lines 5 and 6 separate and parallel from about 1mm from the inner margin, but converging toward the
apex and fused beyond Rs 4 . Submarginal area somewhat greyish, always dark; crossed by the barely visible, parallel first and second submarginal lines. Hindwing upperside: Basal area black, extending along inner margin toward tornus and merging into a dark brown band along the costa. Median area red, reaching neither tornus nor apex. Submarginal band brown, of almost equal width from tornus to apex. Tornal patch greyish, poorly contrasting. Forewing underside: Ground color reddish-yellow, with a thin suffusion of dark grey scales, giving the wing background a granular aspect. Basal area of the forewing grey-beige; first and fourth postmedian lines apparent; the first obvious, the fourth, though thinner, still distinct. Submarginal area pale grey, strongly dentate between M 2 and M 3 . Hindwing underside: Basal area whitish, extending along the costa; submarginal area pale gray, running from apex to vein 2A, where it merges into the basal area. Female and pre-imaginal stages. Unknown.
Male genitalia (Figs. 11a–c). Uncus (Fig. 11a) relatively short and slightly produced dorsally; setigerous lobes distinct; distally somewhat quadrangular, its apex stout and weakly spatulate. Harpe (Fig. 11b) narrow, twisted and strongly bent medially; its apex is somewhat laminated and sclerotized, with an uneven internal margin possibly bent on itself or invaginated; internal margin bearing numerous setae with protruding bases, whereas the ventral side is only slightly setose. Right lobe of the apical process of the aedeagus (Fig. 11c) short and stout, dentate on its internal margin, with only a few, relatively long and unevenly distributed teeth present; left lobe poorly dentate, with very few and barely distinct small teeth.
Distribution: This new species is apparently restricted so far to Guerrero and Michoacán states in Mexico, but is likely also to be present in neighbouring states such as Colima and maybe Jalisco, where very similar and presumably favorable environments exist. It is remarkably isolated from the other Mexican representatives of the X. neoptolemus complex by large desert areas (especially in the Puebla state), and its biotope consists in forested areas with a strong Nearctic influence, clearly contrasting with Neotropical-like forests inhabited by X. cthulhu n. sp. (see below) in southern Mexico.
Genetic variation: The COI sequences for six specimens collected at the same locality in Guerrero state are all identical (Fig. 13 group 2).
Etymology: This species is dedicated to our colleague, Manuel A. Balcázar-Lara (University of Colima, Mexico), for his invaluable assistance to JH in his studies of Mexican sphingids.
Xylophanes cthulhu Haxaire & Vaglia n. sp.
(Figs. 4a, 4b, 12a–c)
Type material: Holotype (Figs. 4a, 4b; deposited in coll. JH; BC-Hax4328/SOWE429-07): m , Guatemala, Izabal department, track from Chocchoc to Cebol (Cevol), km. 2, Pueblo Cadenas, 76 m., 21.vii.2004, leg. O. Paquit & J. Haxaire. Paratypes: 2 m , same data as holotype (BC-Hax4322/SOWE423-07; BC-Hax4324/SOWE425-07); 1 m (BC-Hax4423/SOWE524-07), Guatemala, Succhitepequez Dept., Reserve Tarrales, 1041 m., 7.v.2007, leg. M. Lauras & D. Herbin; 1 m , Guatemala, Baja Verapaz Dept., Reserve Santa Rosa, 1580 m., 23.v.2007, leg. M. Lauras & D. Herbin; 1 m (BC-Hax4329/SOWE430-07), Guatemala, Huehuetenango Dept., Soloma, near Cruz Maltin, Aldea Crinolina, 1900 m., 16.v.2002, leg. J. Monzon Sierra; 1 m (BC-Hax4323/SOWE424-07), Costa Rica, Guanacaste Prov., Area de Conservacion Guanacaste, Sector Pitilla, Estacion Biologica Pitilla, 800m., 4.v.2005, leg. J. Barbut & A. Lévêque; 1 m (BC-Hax0765/SOWA772-06), genit. prep. #Hax171, Mexico, Chiapas State, road from Ococingo to Palenque, track to Salto de Agua, km. 10, 50m., 21.viii.1992, leg. J. Haxaire & D. Herbin; 1 m (BC-Hax0768/SOWA775-06), genit. prep. #Hax169, Mexico, Chiapas State, Municipio Oxchuc, track to Pashtonticja, km. 4, 1600m., 20.viii.1992, leg. J. Haxaire & D. Herbin; 2 m , genit. prep. #Hax168, Mexico, Veracruz State, road from Coatepec-Teocelo to Los Altos, track to Chilchotla, km.4, 1350m., 3.viii.1992, leg. J. Haxaire & D. Herbin; 2 m (BC-Hax0766/SOWA773-06; BC-Hax0767/SOWA-774-06), Mexico, Veracruz State, road from Coatepec-Teocelo to Los Altos, track to Chilchotla, km.4, 1350m., 24.viii.1992, leg. J. Haxaire & D. Herbin; 6 m and 1 w , Mexico, Oaxaca, Sierra Juarez, 12-17.iii.1992, leg. local collectors; 8 m , Mexico, Veracruz, Dos Amates, 20.x.2005, leg. local collectors; 1 w , Mexico, Veracruz, Catemaco, 06.viii.2004, leg. local collectors; 1 m (BC-Hax0770/SOWA777-06), Nicaragua, Granada, Mombacho Volcano, alt. 800 m., 02.viii.2000, leg. M. Laguerre; 3 m , Nicaragua, Nueva Segovia, Rio Mazarite, 10-12.xi.2000, leg. J.-M. Maes; 1 m , Panama, Coclé, Cerro Gaital, El Valle, 27.v.1994, leg. N. Smith & D. Mitchell; 1 m (BC-Hax0769/SOWA776-06), Honduras, Yoro, Pijol Mountain, alt. 1500 m., 19.vii.1995, leg. T. Porion; 2 m , Panama, Panama Prov., Cerro Jofe, 900-1000m, 9-13.v.2007, leg. J. Touroult; 1 & , Panama, Chiriquí, 1980, leg. Moinier; all the above paratypes held in the collections of JH and TV, except 2 m to be deposited in the CNIN and BMNH. In addition, 11 paratypes (6 w , 5 m ) are designated from Costa Rica, Area de Conservacion Guanacaste: 1 m , Sector Cacao, Gongora Bananal, alt. 600 m., 05.viii.2004, leg. M. Pereira; 1 w , Sector Pitilla, Ingas, alt. 580 m., 29.i.2005, leg. M. Rios; 2 m and 2 w , Sector Pitilla, Loaiciga, alt. 445 m., 10-26.i.2005, leg. M. Rios; 2 m and 2 w , Sector Pitilla, Pasmonpa, alt. 440 m., 10-29.i.2005, leg. P. & M. Rios; 1 w , Rincon Rainforest, Camino Rio Francia, alt. 410 m., 16.viii.2004, leg. J.Perez. All of these 11 specimens are part of the dataset used in the DNA barcoding study by Hajibabaei et al. (2006); these specimens to be deposited in the Smithsonian Institution, Washington DC, USA, and specimen data as well as sequences are available from the public BOLD project ‘Sphingidae of the ACG1’ (code MHASA) or GenBank (accession numbers DQ276772 to DQ276782).
Description: This species is immediately distinguishable by its bright coloration and the acute and falcate apex of the forewings. It is widely distributed in Central America and was recorded as X. neoptolemus by Mooser (1940: 456) and Hoffmann (1943: 233) in their surveys of Mexican sphingids.
Male (Figs. 4a, 4b). Head and body: Dorsal part of body olive-brown; labial palpi, area above eyes and tegulae finely marked with grey. Tegula with a median longitudinal gold line. median dorsal area of thorax greyish-beige, contrasting with patagia and tegulae. Upperside of abdomen with five thin longitudinal dark beige lines. Forewing length: 35 mm. Forewing upperside: General background color olive brown; crossed by six postmedian and two submarginal lines as in the previously described species. First and fifth postmedian lines the most heavily contrasted, delimiting between them a cream-colored band in the median area of the wing. General coloration beyond fifth postmedian line remains constant, submarginal area not strongly differentiated from this postmedian part; this region crossed by three even lines (sixth postmedian and the two submarginal). Apex acute, slightly to strongly falcate, especially in specimens from Veracruz State, Mexico. Hindwing upperside: Basal area pure black, extending toward tornus, where it turns pale grey with a white inner edge. Median band bright red, somewhat pinkish in fresher specimens; tapering progressively toward apex of wing, almost reaching it. Forewing and hindwing undersides: Contrasted but with few distinct markings; ground colour reddish yellow, of uniform aspect. Basal area of forewing grey-beige; first and fourth postmedian lines apparent, first straight and strongly marked, fourth very narrow and only visible from inner edge to vein M 2 , beyond which it is replaced by small black vein dots. Between these two lines, the wing is golden-yellow, contrasting with the reddish tone of the rest of the wing; orange submarginal area dentate between M 2 and M 3 in most specimens. First submarginal line also usually apparent, running from the inner margin to the apex.
FIGURE 13. Strict consensus of the nine equally most-parsimonious cladograms (length=133, CI=0.78, RI=0.94) resulting from the phylogenetic analysis of the complete dataset of DNA barcode sequences for 38 specimens belonging to the Xylophanes neoptolemus (yellow) and X. loelia (blue) species complexes (X. cyrene, X. aglaor and X. libya are outgroup taxa). Each specimen is identified by its SampleID code (see Table 1), and the two specimens with short sequences are highlighted in boxes. The branch lengths are proportional to the number of changes (indicated on branches, optimized under FAST optimization); Bremer support and rescaled Bremer support values are given above the branches for each node, and bootstrap support values are indicated below. Clades within the X. neoptolemus complex are named after groups 1, 2 and 3 as described in the text.
Female. Forewing length: 37mm. Identical to the males in terms of general wing pattern and color, both upperside and underside, differing only in the normal differences between sexes in the genus Xylophanes, i.e. larger wingspan, broader and more rounded wings, and thinner antennae.
Male genitalia (Figs. 12a–c). Uncus stout; setigerous lobes developed and distinctly protruding; distal projection bent ventrally, its apex is slightly spatulate and truncate. Harpe short, thick basally and tapering progressively into a thin and slightly upcurved apex. The latter, together with the internal margin of the harpe, only slightly sclerotized. Setae on the harpe few, scattered, and present mostly on the ventral side. Right lobe of apical process of the aedeagus very stout, with long and uneven teeth grouped in the apical portion of its internal margin; left lobe barely distinct, bearing about 10 minute teeth on its lateral part.
Immature stages: A large number of rearing records for this species are reported on the ACG caterpillar inventory website (http://janzen.sas.upenn.edu/); when writing this article, more than 100 pictures of the caterpillar (penultimate and ultimate instars) and pupae of Xylophanes cthulhu were displayed on this site along with detailed collecting information, including food plant identifications. X. cthulhu is reported to feed exclusively on Rubiaceae of the genus Spermacoce L. (52 records on S. exilis, 6 on S. ocymifolia and 1 on S. remota).
Parasitoids: From the numerous rearing records of X. cthulhu in ACG, this species is reported as the host of the parasitoid wasps, Cryptophion inaequalipes (Hymenoptera: Ichneumonidae, Campopleginae. The reported parasitoid actually represents a species complex and a provisional name, C. inaequalipesDHJ02, is currently attached to the specimens reared out of X. cthulhu caterpillars) and Charmedia chavarriai (Ichneumonidae, Ichneumoninae), as well as the tachinid fly, Drino incompta (Diptera: Tachinidae).
Distribution. This species is widely distributed in Central America, inhabiting low to medium altitude forest areas from southern Mexico (Veracruz, Chiapas and Oaxaca states) to eastern Panama (Panama province). A possible contact zone with X. neoptolemus should be searched for in northern Colombia, where specimensof both species might be encountered.
Genetic variation: The lack of genetic variation of this species is remarkable, with a single haplotype found throughout the range from Mexico (Chiapas) to Panama (Fig. 13, group 3).
The following key is primarily based on characters of the habitus, as differences in the genitalia are subtle and diagnosis from the habitus is more rapid and straightforward. Not all differences are listed in this key, and users wishing to investigate further differences between species are invited to use the figures and the more detailed descriptions and discussion of diagnostic characters in the first paragraph of the Results.
The exact congruence between slight but consistent morphological differences and the discrimination of genetic lineages in the two closely related species complexes addressed in this paper is remarkable. As morphology and mitochondrial DNA represent two independently evolving sets of characters, we interpret these results as demonstrating the existence of five distinct lineages, each having its own independent evolutionary trajectory and therefore worthy of being considered as distinct species. The phylogenetic relationships between these species, as inferred through the analysis of available sequences (Fig. 13), show a low level of homoplasy and an excellent resolution, opening interesting perspectives in term of exploring the biogeographical history and course and patterns of diversification of sphingid moths in the Neotropical region.
The authors are grateful to the collectors who made this study possible: J. Barbut, F. Beneluz, P. Bleuzen, J.-N. Carsus, H. Crampette, M. Duranton, D. Herbin, M. Laguerre, M. Lauras, A. Leveque, O. Paquit, J.-Y. Rasplus, S. le Tirant, and B. Vincent. John de Vries provided additional data, and Mehrdad Hajibabaei, Daniel Janzen and Winnie Hallwachs kindly allowed us to access and use the results of the bio-inventory of Lepidoptera in ACG, Costa Rica (project supported by NSF grant DEB0515699) and the related DNA barcoding campaign. For enabling the consultation and research on type specimens, we thank Axel Hausmann (ZSM), Joel Minet and Jacques Pierre (MNHN), Ugo Dall’Asta (RMCA), Willem Hogenes (ZMA), Erik van Nieukerken (NNMN), and Sergey Sinev (ZIRAS). Robert Young (BMNH) kindly produced the digital images of types in the BMNH. Alex Smith and Paul D. N. Hebert kindly helped in editing the manuscript. Rodolphe Rougerie acknowledges support by grants from the Natural Sciences and Engineering Research Council (Canada) and Genome Canada to the Canadian Barcode of Life Network.
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